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nuclear counterstain dapi  (Cytoskeleton Inc)
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vector trueview  (Vector Laboratories)
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6-diamidino-2-phenylindole dihydrochloride (dapi, 99)  (Keygen Biotech)
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dapi (d9542)  (Millipore)
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annexin v-apc/cyanine7/dapi apoptosis kit  (Elabscience Biotechnology)
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1,1′′-dioctadecyl3,3,3′′,3′-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt (did)  (Beyotime)
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dapi  (Beyotime)
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dapi  (Bio-Techne corporation)
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Fluorescent in situ hybridisation for ( a ) known coloboma gene targets and ( b ) OFC candidate genes identified in this <t>study</t> <t>counterstained</t> with <t>DAPI</t> nuclear stain on chick eyes at HH.St28, immediately prior to fusion. Note for EMX2 the OFM region highlights the POM region - expression was not observed in the NR or RPE. In both a and b , Top : grayscale RNAscope probe signal without DAPI highlighting mRNA signal in the immediate optic fissure margin region. Middle: mRNA probe and DAPI merged images of broad ventral eye region. Bottom: Dorsal eye region with probe and DAPI merged. OFM, optic fissure margin; NR, neural retina; POM, periocular mesenchyme; RPE, retinal pigmented epithelium. Scale = 50 μm.
Dapi, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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high resolution dna kit type p solution b  (Partec)
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Partec high resolution dna kit type p solution b
Fluorescent in situ hybridisation for ( a ) known coloboma gene targets and ( b ) OFC candidate genes identified in this <t>study</t> <t>counterstained</t> with <t>DAPI</t> nuclear stain on chick eyes at HH.St28, immediately prior to fusion. Note for EMX2 the OFM region highlights the POM region - expression was not observed in the NR or RPE. In both a and b , Top : grayscale RNAscope probe signal without DAPI highlighting mRNA signal in the immediate optic fissure margin region. Middle: mRNA probe and DAPI merged images of broad ventral eye region. Bottom: Dorsal eye region with probe and DAPI merged. OFM, optic fissure margin; NR, neural retina; POM, periocular mesenchyme; RPE, retinal pigmented epithelium. Scale = 50 μm.
High Resolution Dna Kit Type P Solution B, supplied by Partec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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annexin v-apc/dapi apoptosis kit  (Guangzhou JET Bio-Filtration)
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Guangzhou JET Bio-Filtration annexin v-apc/dapi apoptosis kit
Fluorescent in situ hybridisation for ( a ) known coloboma gene targets and ( b ) OFC candidate genes identified in this <t>study</t> <t>counterstained</t> with <t>DAPI</t> nuclear stain on chick eyes at HH.St28, immediately prior to fusion. Note for EMX2 the OFM region highlights the POM region - expression was not observed in the NR or RPE. In both a and b , Top : grayscale RNAscope probe signal without DAPI highlighting mRNA signal in the immediate optic fissure margin region. Middle: mRNA probe and DAPI merged images of broad ventral eye region. Bottom: Dorsal eye region with probe and DAPI merged. OFM, optic fissure margin; NR, neural retina; POM, periocular mesenchyme; RPE, retinal pigmented epithelium. Scale = 50 μm.
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m o m kit  (Vector Laboratories)
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Vector Laboratories m o m kit
Fluorescent in situ hybridisation for ( a ) known coloboma gene targets and ( b ) OFC candidate genes identified in this <t>study</t> <t>counterstained</t> with <t>DAPI</t> nuclear stain on chick eyes at HH.St28, immediately prior to fusion. Note for EMX2 the OFM region highlights the POM region - expression was not observed in the NR or RPE. In both a and b , Top : grayscale RNAscope probe signal without DAPI highlighting mRNA signal in the immediate optic fissure margin region. Middle: mRNA probe and DAPI merged images of broad ventral eye region. Bottom: Dorsal eye region with probe and DAPI merged. OFM, optic fissure margin; NR, neural retina; POM, periocular mesenchyme; RPE, retinal pigmented epithelium. Scale = 50 μm.
M O M Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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4 6 diamidino 2 phenylindole dihydrochloride dapi  (Thermo Fisher)
99
Thermo Fisher 4 6 diamidino 2 phenylindole dihydrochloride dapi
Fluorescent in situ hybridisation for ( a ) known coloboma gene targets and ( b ) OFC candidate genes identified in this <t>study</t> <t>counterstained</t> with <t>DAPI</t> nuclear stain on chick eyes at HH.St28, immediately prior to fusion. Note for EMX2 the OFM region highlights the POM region - expression was not observed in the NR or RPE. In both a and b , Top : grayscale RNAscope probe signal without DAPI highlighting mRNA signal in the immediate optic fissure margin region. Middle: mRNA probe and DAPI merged images of broad ventral eye region. Bottom: Dorsal eye region with probe and DAPI merged. OFM, optic fissure margin; NR, neural retina; POM, periocular mesenchyme; RPE, retinal pigmented epithelium. Scale = 50 μm.
4 6 Diamidino 2 Phenylindole Dihydrochloride Dapi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fluorescent in situ hybridisation for ( a ) known coloboma gene targets and ( b ) OFC candidate genes identified in this study counterstained with DAPI nuclear stain on chick eyes at HH.St28, immediately prior to fusion. Note for EMX2 the OFM region highlights the POM region - expression was not observed in the NR or RPE. In both a and b , Top : grayscale RNAscope probe signal without DAPI highlighting mRNA signal in the immediate optic fissure margin region. Middle: mRNA probe and DAPI merged images of broad ventral eye region. Bottom: Dorsal eye region with probe and DAPI merged. OFM, optic fissure margin; NR, neural retina; POM, periocular mesenchyme; RPE, retinal pigmented epithelium. Scale = 50 μm.

Journal: bioRxiv

Article Title: Identification of novel coloboma candidate genes through conserved gene expression analyses across four vertebrate species

doi: 10.1101/2022.12.22.521600

Figure Lengend Snippet: Fluorescent in situ hybridisation for ( a ) known coloboma gene targets and ( b ) OFC candidate genes identified in this study counterstained with DAPI nuclear stain on chick eyes at HH.St28, immediately prior to fusion. Note for EMX2 the OFM region highlights the POM region - expression was not observed in the NR or RPE. In both a and b , Top : grayscale RNAscope probe signal without DAPI highlighting mRNA signal in the immediate optic fissure margin region. Middle: mRNA probe and DAPI merged images of broad ventral eye region. Bottom: Dorsal eye region with probe and DAPI merged. OFM, optic fissure margin; NR, neural retina; POM, periocular mesenchyme; RPE, retinal pigmented epithelium. Scale = 50 μm.

Article Snippet: Slides were then mounted in Fluorsave (Merck #345789) with and counterstained with DAPI (ACD Bio-techne, RNAscope Multiplex Fluorescent Reagent Kit v2 #323100).

Techniques: In Situ, Hybridization, Staining, Expressing